ZytoFast®

Genel Bilgi

ZytoFast® ürünleri formalin-fikse, parafine gömülü doku kesitlerinde ve hücre örneklerinde Kromojenik in situ Hibridizasyon (CISH) yoluyla HPV, EBV, CMV gibi insan patojen virüslerinin olağanüstü hızlı bir şekilde tespit edilmesi ve ayırt edilmesi ve Ig-κ ve Ig-λ hafif zincir RNA’yı tespit ederek lenfosit klon durumunun belirlenmesi için tasarlanmıştır. ZytoFast® problarının sinyal yoğunluğu ZytoFast® PLUS Uygulama Kitlerinin kullanıldığında daha da artar.

Yöntemin Tanımı – ZytoFast®

ZytoFast® sisteminde Biyotin eklenmiş oligonükleotid problar kullanılır. Bunlar biyotin eklentisini hedef alan enzim-konjuge streptavidin kullanılarak tespit edilir. BCIP/NBT veya AEC gibi kromojenik substratların enzimatik reaksiyonları ışık mikroskobunda görüntülenebilen güçlü renk çökeltilerinin meydana gelmesine yol açar.

Yöntemin Tanımı – ZytoFast® PLUS

ZytoFast® PLUS sisteminde primer antikorlar ile tespit edilen Digoxigenin işaretli problar kullanılır. bu antikorlar polimerize enzim-konjuge sekonder antikorlar tarafından tespit edilir. NBT/BCIP, AEC veya DAB gibi kromojenik substratların enzimatik reaksiyonu ışık mikroskobunda görüntülenebilen güçlü renk çökeltilerinin meydana gelmesine yol açar.

ZytoFast® – İhtiyaçlarınıza cevap veren esneklik

  • Tüm Biyotin işaretli problar ZytoFast® CISH Uygulama Kiti ile birlikte kullanılabilir.
  • Tüm Digoxigenin işaretli problar ZytoFast® PLUS CISH Uygulama Kiti ile birlikte kullanılabilir.

Virus Index

HPV

Digoxigenin-labeled

ZytoFast® HPV type 6/11 Probe
T-1055-400
ZytoFast® HPV type 16/18 Probe
T-1056-400
ZytoFast® HPV type 31/33 Probe
T-1057-400
ZytoFast® HPV High-Risk (HR) Types Probe
(specific for HPV type 16/18/31/33/35/39/45/51/52/56/58/59/66/68/82)
T-1140-400
ZytoFast® HPV Screening Probe
(specific for HPV type 6/11/16/18/31/33/35/39/45/51/52/56/58/59/66/68/82)
T-1144-400

EBV (a.k.a. HHV-4)

Digoxigenin-labeled

ZytoFast® EBV Probe
T-1114-400

CMV (a.k.a. HHV-5)

Digoxigenin-labeled

ZytoFast® CMV Probe
T-1113-400

RNA Index

Ig-kappa

Digoxigenin-labeled

ZytoFast® human lg-kappa Probe
T-1115-400

Digoxigenin/Biotin-labeled

ZytoFast® human lg-kappa/lg-lambda Probe
T-1017-400
ZytoFast® human lg-kappa/lg-lambda CISH Kit
T-1005-40
ZytoFast® human lg-kappa/lg-lambda Permanent CISH Kit
T-1105-40

Ig-lambda

Digoxigenin-labeled

ZytoFast® human lg-lambda Probe
T-1116-400

Digoxigenin/Biotin-labeled

ZytoFast® human lg-kappa/lg-lambda Probe
T-1017-400
ZytoFast® human lg-kappa/lg-lambda CISH Kit
T-1005-40
ZytoFast® human lg-kappa/lg-lambda Permanent CISH Kit
T-1105-40

Endikasyon İndeksi

Solid Tümörler

Serviks Cancer

Digoxigenin-labeled

ZytoFast® HPV type 6/11 Probe
T-1055-400
ZytoFast® HPV type 16/18 Probe
T-1056-400
ZytoFast® HPV type 31/33 Probe
T-1057-400
ZytoFast® HPV High-Risk (HR) Types Probe
(specific for HPV type 16/18/31/33/35/39/45/51/52/56/58/59/66/68/82)
T-1140-400
ZytoFast® HPV Screening Probe
(specific for HPV type 6/11/16/18/31/33/35/39/45/51/52/56/58/59/66/68/82)
T-1144-400

Hematolojiye Özel Problar

Lenfoma, diğer

Digoxigenin-labeled

ZytoFast® EBV Probe
T-1114-400
ZytoDot® human Ig-kappa Probe
T-1115-400
ZytoDot® CEN Yq12 Probe ZytoFast® human Ig-lambda Probe
T-1116-400

Digoxigenin/Biotin-labeled

ZytoFast® human Ig-kappa/Ig-lambda Probe
T-1017-400
ZytoDot® human Ig-kappa/Ig-lambda CISH Kit
T-1005-40
ZytoFast® human lg-kappa/lg-lambda Permanent CISH Kit
T-1105-40

Aksesuarlar

ZytoFast® PLUS Implementation Kits

For the Detection of Digoxigenin-labeled ZytoFast® Probes

ZytoFast® PLUS CISH Implementation Kit AP-NBT/BCIP
T-1061-40
ZytoFast® PLUS CISH Implementation Kit HRP-DAB
T-1063-40
ZytoFast® PLUS CISH Implementation Kit AP-Permanent Red
T-1151-40

ZytoFast® Pretreatment Reagents

Pepsin Solution
ES-0001-4/-8/-50/-1000
Heat Pretreatment Solution EDTA
PT-0002-500

ZytoFast® Wash Buffers & Ancillary Reagents

Mouse-anti-DIG
AB-0001-4/-30
Anti-Mouse-HRP-Polymer
AB-0002-4
Rabbit-anti-DIG
AB-0011-4
Anti-Biotin/DIG-Mix
AB-0015-4
DAB Solution Set
C-3015-100
Zyto Dot® HRP-Green Solution Set
AB-0013-4C-3039-100 su_column]
Mayer’s Hematoxylin Solution
CS-0001-20
Nuclear Blue Solution
CS-0002-20
Nuclear Green Solution
CS-0004-20
Nuclear Red Solution
CS-0003-20
Mounting Solution (alcoholic)
MT-0004-4
NBT/BCIP Solution
SB-0004-4
AEC Solution
SB-0005-4
20x Wash Buffer TBS
WB-0005-50

ZytoFast® Wash Buffers & Ancillary Reagents

Zyto Fast® DNA (+) Control Probe
T-1053-400
Zyto Fast® DNA (-) Control Probe
T-1054-400
Zyto Fast® 28S rRNA (+) Control Probe
T-1120-400
Zyto Fast® RNA (-) Control Probe
T-1119-400