FlexISH®
Genel Bilgi
FlexISH® ürünleri kromozomal bozuklukların çeşitli örneklerde Floresan in situ Hibridizasyon (FISH) ile tespit edlimesi için tasarlanmıştır. FlexISH® ürünleri kişisel ihtiyaçlarınıza göre hibridizasyon süresini değiştirerek 1 günlük (2 saat hübridizasyon) veya 2 günlük (gece boyu hibridizasyon) protokoller arasında seçim yapma esnekliği sunar!
FlexISH® FISH uygulamalarınıza esneklik kazandırır
- FlexISH® zaman ve laboratuvar yönetimi bakımından esnekliğinizi en yüksek seviyeye çıkarır. Hibridizasyon süresi 2 saat ile gece boyu arasında değiştirilebilir.
- 37°C hibridizasyon sıcaklığı ile FlexISH® protokolü patoloji laboratuvarlarının rutin iş akışına tamamen uygundur.
- Yakın zamanlı bir karşılaştırma çalışmasında gece boyu ve kısa hibridizasyon süreleri FlexISH®, ZytoLight® ve PathVysion ERBB2 probları ile elde edilen FISH sonuçları arasında mükemmel korelasyon gösterdi1.
- Kısa hibridizasyon süresi performansı, örnek kalitesini veya tanı sonucunu etkilemez 1.
Yöntemin Tanımı ve FlexISH® Florokromları
ZytoLight® sisteminde doğrudan işaretli FISH probları kullanılır. Problar floresan mikroskobunda uygun filtre setleri kullanılarak görüntülenir. Mikroskobun lambasından çıkan tam-spektrumlu ışık, uyarıcı (exiter) filtre sayesinde spesifik olarak probun floroforunu uyarmak için tanımlanan dalga boyuna indirgenir. Bu ışık bir dikronik aynadan örneğin üzerine yansıtılır. Florofor, aynadan geçen daha uzun dalga boylu ışık yayar. Son olarak, bir bariyer filtre yayılan ışığı önceden tanımlanmış görüntülenebilir bir dalga boyuna indirger.
FISH analizlerini esas olarak iki faktör etkiler:
- FISH problarının florokromları
- Uygun filtre setleri
Uygun filtre setleri hakkında net bilgi almak için lütfen yerel dağıtıcınız ile iletişime geçiniz.
FlexISH® Kitleri – Elverişli Çözümler
Tüm FlexISH® FISH probları
- FFPE örneklerde FISH analizleri için FlexISH-Doku Uygulama Kiti (Z-2182-5/-20) İle birlikte kullanılabilir.
Zytolight Kromozom List
-
ZytoLight® Glioma 1p/19q Probe Set
-
ZytoLight® SPEC 1p12 Probe
-
ZytoLight® SPEC 1p36/1q25 Dual Color Probe
-
ZytoLight® SPEC ABL2 Dual Color Break Apart Probe
-
ZytoLight® SPEC CKS1B/CDKN2C Dual Color Probe
-
ZytoLight® SPEC FOXO1/PAX7 Dual Color Single Fusion Probe
-
ZytoLight® SPEC MCL1/1p12 Dual Color Probe
-
ZytoLight® SPEC MDM4/1p12 Dual Color Probe
-
ZytoLight® SPEC MEF2D/BCL9 TriCheck™ Probe
-
ZytoLight® SPEC NTRK1 Dual Color Break Apart Probe
-
ZytoLight® SPEC VHL/1p12/CEN 7/17 Quadruple Color Probe
-
ZytoLight® SPEC 2q11 Probe
-
ZytoLight® SPEC ALK/EML4 TriCheckTM Probe
-
span class="textleft">ZytoLight® SPEC ALK Dual Color Break Apart Probe
-
ZytoLight® SPEC ALK/2q11 Dual Color Probe
-
ZytoLight® SPEC CCND1 Break Apart/2q11/CEN 6 Quadruple Color Probe
-
ZytoLight® SPEC EML4 Dual Color Break Apart Probe
-
ZytoLight® SPEC ERBB4/2q11 Dual Color Probe
-
ZytoLight® SPEC FOXO1/PAX3 Dual Color Single Fusion Probe
-
ZytoLight® SPEC FOXO1/PAX3 TriCheckTM Probe
-
ZytoLight® SPEC IGK Break Apart Probe
-
ZytoLight® SPEC MYCN/2q11 Dual Color Probe
-
ZytoLight® CEN 3 Probe
-
ZytoLight® SPEC BCL6 Dual Color Break Apart Probe
-
ZytoLight® SPEC CDKN2A/CEN 3/7/17 Quadruple Color Probe
-
ZytoLight® SPEC FHIT/CEN 3 Dual Color Probe
-
ZytoLight® SPEC GATA2/MECOM Dual Color Dual Fusion Probe
-
ZytoLight® SPEC PIK3CA/CEN 3 Dual Color Probe
-
ZytoLight® SPEC SOX2/CEN 3 Dual Color Probe
-
ZytoLight® SPEC TERC/CEN 3 Dual Color Probe
-
ZytoLight® SPEC VHL/1p12/CEN 7/17 Quadruple Color Probe
-
ZytoLight® SPEC VHL/CEN 3 Dual Color Probe
-
ZytoLight® SPEC WWTR1 Dual Color Break Apart Probe
-
ZytoLight® SPEC CSF1R Dual Color Break Apart Probe
-
ZytoLight® SPEC CSF1R/D5S23,D5S721 Dual Color Probe
-
ZytoLight® SPEC EGR1/5p15 Dual Color Probe
-
ZytoLight® SPEC EGR1/D5S23,D5S721 Dual Color Probe
-
ZytoLight® SPEC NRG1/CD74 TriCheckTM Probe
-
ZytoLight® SPEC PDGFRB Dual Color Break Apart Probe
-
ZytoLight® SPEC RICTOR/5q31.1 Dual Color Probe
-
ZytoLight® SPEC TERT Dual Color Break Apart
-
ZytoLight® SPEC TERT/5q31 Dual Color Probe
-
ZytoLight® CEN 6 Probe
-
ZytoLight® SPEC CCND1 Break Apart/2q11/CEN 6 Quadruple Color Probe
-
ZytoLight® SPEC ESR1/CEN 6 Dual Color Probe
-
ZytoLight® SPEC IRF4,DUSP22 Dual Color Break Apart Probe
-
ZytoLight® SPEC MYB Dual Color Break Apart Probe
-
ZytoLight® SPEC MYB/CEN 6 Dual Color Probe
-
ZytoLight® SPEC PHF1 Dual Color Break Apart Probe
-
ZytoLight® SPEC ROS1 Dual Color Break Apart Probe
-
ZytoLight® SPEC ROS1/CEN 6 Dual Color Probe
-
ZytoLight® SPEC RREB1/MYB/CEN 6 Triple Color Probe
-
ZytoLight® SPEC VEGFA/CEN 6 Dual Color Probe
-
ZytoLight® CEN 7 Probe
-
ZytoLight® SPEC BRAF Dual Color Break Apart Probe
-
ZytoLight® SPEC BRAF/CEN 7 Dual Color Probe
-
ZytoLight® SPEC CDKN2A/CEN 3/7/17 Quadruple Color Probe
-
ZytoLight® SPEC CUX1/EZH2/CEN 7 Triple Color Probe
-
ZytoLight® SPEC EGFR/CEN 7 Dual Color Probe
-
ZytoLight® SPEC JAZF1 Dual Color Break Apart Probe
-
ZytoLight® SPEC MET/CEN 7 Dual Color Probe
-
ZytoLight® SPEC VHL/1p12/CEN 7/17 Quadruple Color Probe
-
ZytoLight® CEN 8 Probe
-
ZytoLight® SPEC FGFR1 Dual Color Break Apart Probe
-
ZytoLight® SPEC FGFR1/CEN 8 Dual Color Probe
-
ZytoLight® SPEC MYC Dual Color Break Apart Probe
-
ZytoLight® SPEC MYC/CEN 8 Dual Color Probe
-
ZytoLight® SPEC MYC/IGH Dual Color Dual Fusion Probe
-
ZytoLight® SPEC NRG1 Dual Color Break Apart Probe
-
ZytoLight® SPEC NRG1/CD74 TriCheckTM Probe
-
ZytoLight® SPEC RUNX1/RUNX1T1 Dual Color Dual Fusion Probe
-
ZytoLight® CEN 9 Probe
-
ZytoLight® SPEC ABL1 Dual Color Break Apart Probe
-
ZytoLight® SPEC BCR/ABL1 Dual Color Dual Fusion Probe
-
ZytoLight® SPEC CD274,PDCD1LG2/CEN 9 Dual Color Probe
-
ZytoLight® SPEC CDKN2A/CEN 3/7/17 Quadruple Color Probe
-
ZytoLight® SPEC CDKN2A/CEN 9 Dual Color Probe
-
ZytoLight® SPEC JAK2 Dual Color Break Apart Probe
-
ZytoLight® SPEC NR4A3 Dual Color Break Apart Probe
-
ZytoLight® SPEC NTRK2 Dual Color Break Apart Probe
-
ZytoLight® SPEC NUP214 Dual Color Break Apart Probe
-
ZytoLight® CEN 11 Probe
-
ZytoLight® SPEC 11q gain/loss Triple Color Probe
-
ZytoLight® SPEC ATM/CEN 11 Dual Color Probe
-
ZytoLight® SPEC ATM/CEN 12 Dual Color Probe
-
ZytoLight® SPEC BIRC3/MALT1 Dual Color Dual Fusion Probe
-
ZytoLight® SPEC CARS Dual Color Break Apart Probe
-
ZytoLight® SPEC CCND1 Break Apart/2q11/CEN 6 Quadruple Color Probe
-
ZytoLight® SPEC CCND1 Dual Color Break Apart Probe
-
ZytoLight® SPEC CCND1/CEN 11 Dual Color Probe
-
ZytoLight® SPEC CCND1/IGH Dual Color Dual Fusion Probe
-
ZytoLight® SPEC EWSR1/FLI1 TriCheckTM Probe
-
ZytoLight® SPEC KMT2A Dual Color Break Apart Probe
-
ZytoLight® SPEC MAML2 Dual Color Break Apart Probe
-
ZytoLight® SPEC NUP98 Dual Color Break Apart Probe
-
ZytoLight® SPEC SPI1 Dual Color Break Apart Probe
-
ZytoLight® SPEC TP53/ATM Dual Color Probe
-
ZytoLight® SPEC WT1 Dual Color Break Apart Probe
-
ZytoLight® CEN 12 Probe
-
ZytoLight® SPEC ATM/CEN 12 Dual Color Probe
-
ZytoLight® SPEC CDK4/CEN 12 Dual Color Probe
-
ZytoLight® SPEC D13S319/13q34/CEN 12 Triple Color Probe
-
ZytoLight® SPEC DDIT3 Dual Color Break Apart Probe
-
ZytoLight® SPEC ERBB3/CEN 12 Dual Color Probe
-
ZytoLight® SPEC ETV6 Dual Color Break Apart Probe
-
ZytoLight® SPEC ETV6/RUNX1 Dual Color Dual Fusion Probe
-
ZytoLight® SPEC KRAS/CEN 12 Dual Color Probe
-
ZytoLight® SPEC MDM2/CEN 12 Dual Color Probe
-
ZytoLight® SPEC ZNF384 Dual Color Break Apart Probe
-
ZytoLight® Aneuploidy Panel X/Y and 13/18/21
-
ZytoLight® Aneuploidy Panel 18/X/Y and 13/21
-
ZytoLight® SPEC 13q12 Probe
-
ZytoLight® SPEC 13/21 Dual Color Probe
-
ZytoLight® SPEC 13/CEN 18/SPEC 21 Triple Color Probe
-
ZytoLight® SPEC D13S319/13q34/CEN 12 Triple Color Probe
-
ZytoLight® SPEC D13S319/13q34 Dual Color Probe
-
ZytoLight® SPEC FOXO1 Dual Color Break Apart Probe
-
ZytoLight® SPEC FOXO1/PAX3 Dual Color Single Fusion Probe
-
ZytoLight® SPEC FOXO1/PAX3 TriCheckTM Probe
-
ZytoLight® SPEC FOXO1/PAX7 Dual Color Single Fusion Probe
-
ZytoLight® SPEC RB1/13q12 Dual Color Probe
-
ZytoLight® SPEC BCL2/IGH Dual Color Dual Fusion Probe
-
ZytoLight® SPEC CCND1/IGH Dual Color Dual Fusion Probe
-
ZytoLight® SPEC FGFR3/IGH Dual Color Dual Fusion Probe
-
ZytoLight® SPEC IGH Dual Color Break Apart Probe
-
ZytoLight® SPEC MAF/IGH Dual Color Dual Fusion Probe
-
ZytoLight® SPEC MAFB/IGH Dual Color Dual Fusion Probe
-
ZytoLight® SPEC MYC/IGH Dual Color Dual Fusion Probe
-
ZytoLight® CEN 17 Probe
-
ZytoLight® CEN 17/SPEC ERBB2 Dual Color Probe
-
ZytoLight® SPEC CDKN2A/CEN 3/7/17 Quadruple Color Probe
-
ZytoLight® SPEC COL1A1 Dual Color Break Apart Probe
-
ZytoLight® SPEC COL1A1/PDGFB Dual Color Dual Fusion Probe
-
ZytoLight® SPEC ERBB2/CEN 17 Dual Color Probe
-
ZytoLight® SPEC ERBB2/CEN 17 Dual Color Probe Kit
-
ZytoLight® SPEC ERBB2/D17S122 Dual Color Probe
-
ZytoLight® SPEC ERBB2/TOP2A/CEN 17 Triple Color Probe
-
ZytoLight® SPEC PML/RARA Dual Color Dual Fusion Probe
-
span class="textleft">ZytoLight® SPEC TP53/17q22 Dual Color Probe
-
ZytoLight® SPEC TP53/ATM Dual Color Probe
-
ZytoLight® SPEC TP53/CEN 17 Dual Color Probe
-
ZytoLight® SPEC USP6 Dual Color Break Apart Probe
-
ZytoLight® SPEC VHL/1p12/CEN 7/17 Quadruple Color Probe
-
ZytoLight® SPEC YWHAE Dual Color Break Apart Probe
-
ZytoLight® Aneuploidy Panel X/Y and 13/18/21
-
ZytoLight® Aneuploidy Panel 18/X/Y and 13/21
-
ZytoLight® CEN 18 Probe
-
ZytoLight® SPEC 13/CEN 18/SPEC 21 Triple Color Probe
-
ZytoLight® SPEC 18/CEN X/Y Triple Color Probe
-
ZytoLight® SPEC BCL2 Dual Color Break Apart Probe
-
ZytoLight® SPEC BCL2/CEN 18 Dual Color Probe
-
ZytoLight® SPEC BCL2/IGH Dual Color Dual Fusion Probe
-
ZytoLight® SPEC BIRC3/MALT1 Dual Color Dual Fusion Probe
-
ZytoLight® SPEC MALT1 Dual Color Break Apart Probe
-
ZytoLight® SPEC SS18 Dual Color Break Apart Probe
-
ZytoLight® SPEC SS18/SSX1 TriCheckTM Probe
-
ZytoLight® Aneuploidy Panel X/Y and 13/18/21
-
ZytoLight® Aneuploidy Panel 18/X/Y and 13/21
-
ZytoLight® SPEC 13/21 Dual Color Probe
-
ZytoLight® SPEC 13/CEN 18/SPEC 21 Triple Color Probe
-
ZytoLight® SPEC 21q22 Probe
-
ZytoLight® SPEC 21/CEN X/Yq12 Triple Color Probe
-
ZytoLight® SPEC ERG Dual Color Break Apart Probe
-
ZytoLight® SPEC ERG/TMPRSS2 TriCheckTM Probe
-
ZytoLight® SPEC ETV6/RUNX1 Dual Color Dual Fusion Probe
-
ZytoLight® SPEC RUNX1/RUNX1T1 Dual Color Dual Fusion Probe
-
ZytoLight® SPEC BCR/ABL1 Dual Color Dual Fusion Probe
-
ZytoLight® SPEC COL1A1/PDGFB Dual Color Dual Fusion Probe
-
ZytoLight® SPEC DiGeorge/Phelan McDermid Dual Color Probe
-
ZytoLight® SPEC DiGeorge Triple Color Probe
-
ZytoLight® SPEC EWSR1 Dual Color Break Apart Probe
-
ZytoLight® SPEC EWSR1/FLI1 TriCheckTM Probe
-
ZytoLight® SPEC IGL Dual Color Break Apart Probe
-
ZytoLight® SPEC PDGFB Dual Color Break Apart Probe
-
ZytoLight® SPEC SMARCB1/22q12 Dual Color Probe
-
ZytoLight® Aneuploidy Panel X/Y and 13/18/21
-
ZytoLight® Aneuploidy Panel 18/X/Y and 13/21
-
ZytoLight® CEN X Probe
-
ZytoLight® CEN X/Y Dual Color Probe
-
ZytoLight® CEN X/Yq12 Dual Color Probe
-
ZytoLight® SPEC 18/CEN X/Y Triple Color Probe
-
ZytoLight® SPEC 21/CEN X/Yq12 Triple Color Probe
-
ZytoLight® SPEC CRLF2 Dual Color Break Apart Probe
-
ZytoLight® SPEC SS18/SSX1 TriCheckTM Probe
-
ZytoLight® SPEC TFE3 Dual Color Break Apart Probe
-
ZytoLight® Aneuploidy Panel X/Y and 13/18/21
-
ZytoLight® Aneuploidy Panel 18/X/Y and 13/21
-
ZytoLight® CEN X/Y Dual Color Probe
-
ZytoLight® CEN X/Yq12 Dual Color Probe
-
ZytoLight® CEN Y (DYZ3) Probe
-
ZytoLight® CEN Yq12 Probe
-
ZytoLight® SPEC 18/CEN X/Y Triple Color Probe
-
ZytoLight® SPEC 21/CEN X/Yq12 Triple Color Probe
-
ZytoLight® SPEC CRLF2 Dual Color Break Apart Probe